Isolation of Different Animal Liver Xanthine Oxidase Containing Fractions and Determination of Kinetic Parameters for Xanthine
نویسنده
چکیده
Xanthine oxidase activity containing fractions from rat, mouse, rabbit and guinea pig livers were obtained by heat treatment and ammonium sulfate precipitation. Xanthine oxidase activity was observed in rat and mouse liver fractions, while xanthine oxidase activity was absent in rabbit and guinea pig liver fractions. Enzyme kinetic parameters, K m and V max , were determined for the conversion of xanthine to uric acid by rat and mouse live fractions, by both spectrophotometric and HPLC methods. The K m values obtained by either method for both animal liver fractions were in the range of 5.32-13.8 μM.
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